First, there is no need to use the Ubuntu persistent file setting. It may or may not even be usable in LiveCDs that stray from the herd. I don't know.
There are new menu options in Navigatrix to make make larger persistent file creation easier. Hit <anykey> at boot (when it does boot) and select
other options.
What sort of boot loader error are you getting?
SYSLINUX 4.06 EDD 4.06-pre7 Copyright (C) 1994-2012 H. Peter Anvin et al only or the
"Busy Box" with "initramf" error
In the meantime check (and repair) the integrity of the file system on the stick
sudo dosfsck -a -l -t -v -w /dev/sdX
1 ...with
X being the location (a,b,c,d...) of your stick.
That's as
Super
User
DO DOS File
System
Chec
K -a automatically repair the file system [easy]
-l list path names [informative]
-t test for bad clusters [healthy]
-v verbose mode [fun]
-w write changes to disk immediately [satisfying]
on
/dev/sdX1The application
dosfsck while standard in Navigatrix might not be in Ubuntu. If it not, obtain it with
sudo apt-get install dosfstoolsRun that to see (and fix) if there is something buggered on your stick.
Also, is this a Sandisc USB stick, by the way?